Study Summary
This study compared viable lyopreserved AM (VLAM) to VCAM and fresh AM using in vitro and in vivo models. While viable cryopreserved amnion (VCAM) retains these beneficial components and has shown success in wound care, its reliance on ultra-low temperature storage limits accessibility. Findings indicate VLAM retains a structure, growth factors and cell viability similar to VCAM and fresh AM, and both VLAM and VCAM reduced TNF-α secretion, increased VEGF expression under inflammatory and hypoxic conditions and promoted wound closure in a diabetic mouse model.